The life cycle of SARS coronavirus in Vero E6 cells.
Identifieur interne : 005015 ( Main/Exploration ); précédent : 005014; suivant : 005016The life cycle of SARS coronavirus in Vero E6 cells.
Auteurs : Zhang Qinfen [République populaire de Chine] ; Cui Jinming ; Huang Xiaojun ; Zheng Huanying ; Huang Jicheng ; Fang Ling ; Li Kunpeng ; Zhang JingqiangSource :
- Journal of medical virology [ 0146-6615 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Appareil de Golgi (virologie), Assemblage viral, Cellules Vero, Fusion membranaire, Membrane cellulaire (virologie), Microscopie électronique, Noyau de la cellule (virologie), Réplication virale, Réticulum endoplasmique rugueux (virologie), Virus du SRAS (croissance et développement), Virus du SRAS (pathogénicité), Vésicules cytoplasmiques (virologie).
- MESH :
- croissance et développement : Virus du SRAS.
- pathogénicité : Virus du SRAS.
- virologie : Appareil de Golgi, Membrane cellulaire, Noyau de la cellule, Réticulum endoplasmique rugueux, Vésicules cytoplasmiques.
- Animaux, Assemblage viral, Cellules Vero, Fusion membranaire, Microscopie électronique, Réplication virale.
English descriptors
- KwdEn :
- Animals, Cell Membrane (virology), Cell Nucleus (virology), Chlorocebus aethiops, Cytoplasmic Vesicles (virology), Endoplasmic Reticulum, Rough (virology), Golgi Apparatus (virology), Membrane Fusion, Microscopy, Electron, SARS Virus (growth & development), SARS Virus (pathogenicity), Vero Cells, Virus Assembly, Virus Replication.
- MESH :
- growth & development : SARS Virus.
- pathogenicity : SARS Virus.
- virology : Cell Membrane, Cell Nucleus, Cytoplasmic Vesicles, Endoplasmic Reticulum, Rough, Golgi Apparatus.
- Animals, Chlorocebus aethiops, Membrane Fusion, Microscopy, Electron, Vero Cells, Virus Assembly, Virus Replication.
Abstract
The aim of the study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.
DOI: 10.1002/jmv.20095
PubMed: 15170625
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002D67
- to stream PubMed, to step Curation: 002D67
- to stream PubMed, to step Checkpoint: 002983
- to stream Ncbi, to step Merge: 000839
- to stream Ncbi, to step Curation: 000839
- to stream Ncbi, to step Checkpoint: 000839
- to stream Main, to step Merge: 005376
- to stream Main, to step Curation: 005015
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The life cycle of SARS coronavirus in Vero E6 cells.</title><author><name sortKey="Qinfen, Zhang" sort="Qinfen, Zhang" uniqKey="Qinfen Z" first="Zhang" last="Qinfen">Zhang Qinfen</name><affiliation wicri:level="3"><nlm:affiliation>State Key Lab for Biocontrol, Zhongshan University, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Lab for Biocontrol, Zhongshan University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Jinming, Cui" sort="Jinming, Cui" uniqKey="Jinming C" first="Cui" last="Jinming">Cui Jinming</name></author><author><name sortKey="Xiaojun, Huang" sort="Xiaojun, Huang" uniqKey="Xiaojun H" first="Huang" last="Xiaojun">Huang Xiaojun</name></author><author><name sortKey="Huanying, Zheng" sort="Huanying, Zheng" uniqKey="Huanying Z" first="Zheng" last="Huanying">Zheng Huanying</name></author><author><name sortKey="Jicheng, Huang" sort="Jicheng, Huang" uniqKey="Jicheng H" first="Huang" last="Jicheng">Huang Jicheng</name></author><author><name sortKey="Ling, Fang" sort="Ling, Fang" uniqKey="Ling F" first="Fang" last="Ling">Fang Ling</name></author><author><name sortKey="Kunpeng, Li" sort="Kunpeng, Li" uniqKey="Kunpeng L" first="Li" last="Kunpeng">Li Kunpeng</name></author><author><name sortKey="Jingqiang, Zhang" sort="Jingqiang, Zhang" uniqKey="Jingqiang Z" first="Zhang" last="Jingqiang">Zhang Jingqiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15170625</idno><idno type="pmid">15170625</idno><idno type="doi">10.1002/jmv.20095</idno><idno type="wicri:Area/PubMed/Corpus">002D67</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D67</idno><idno type="wicri:Area/PubMed/Curation">002D67</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002D67</idno><idno type="wicri:Area/PubMed/Checkpoint">002983</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002983</idno><idno type="wicri:Area/Ncbi/Merge">000839</idno><idno type="wicri:Area/Ncbi/Curation">000839</idno><idno type="wicri:Area/Ncbi/Checkpoint">000839</idno><idno type="wicri:doubleKey">0146-6615:2004:Qinfen Z:the:life:cycle</idno><idno type="wicri:Area/Main/Merge">005376</idno><idno type="wicri:Area/Main/Curation">005015</idno><idno type="wicri:Area/Main/Exploration">005015</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The life cycle of SARS coronavirus in Vero E6 cells.</title><author><name sortKey="Qinfen, Zhang" sort="Qinfen, Zhang" uniqKey="Qinfen Z" first="Zhang" last="Qinfen">Zhang Qinfen</name><affiliation wicri:level="3"><nlm:affiliation>State Key Lab for Biocontrol, Zhongshan University, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Lab for Biocontrol, Zhongshan University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Jinming, Cui" sort="Jinming, Cui" uniqKey="Jinming C" first="Cui" last="Jinming">Cui Jinming</name></author><author><name sortKey="Xiaojun, Huang" sort="Xiaojun, Huang" uniqKey="Xiaojun H" first="Huang" last="Xiaojun">Huang Xiaojun</name></author><author><name sortKey="Huanying, Zheng" sort="Huanying, Zheng" uniqKey="Huanying Z" first="Zheng" last="Huanying">Zheng Huanying</name></author><author><name sortKey="Jicheng, Huang" sort="Jicheng, Huang" uniqKey="Jicheng H" first="Huang" last="Jicheng">Huang Jicheng</name></author><author><name sortKey="Ling, Fang" sort="Ling, Fang" uniqKey="Ling F" first="Fang" last="Ling">Fang Ling</name></author><author><name sortKey="Kunpeng, Li" sort="Kunpeng, Li" uniqKey="Kunpeng L" first="Li" last="Kunpeng">Li Kunpeng</name></author><author><name sortKey="Jingqiang, Zhang" sort="Jingqiang, Zhang" uniqKey="Jingqiang Z" first="Zhang" last="Jingqiang">Zhang Jingqiang</name></author></analytic><series><title level="j">Journal of medical virology</title><idno type="ISSN">0146-6615</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Membrane (virology)</term><term>Cell Nucleus (virology)</term><term>Chlorocebus aethiops</term><term>Cytoplasmic Vesicles (virology)</term><term>Endoplasmic Reticulum, Rough (virology)</term><term>Golgi Apparatus (virology)</term><term>Membrane Fusion</term><term>Microscopy, Electron</term><term>SARS Virus (growth & development)</term><term>SARS Virus (pathogenicity)</term><term>Vero Cells</term><term>Virus Assembly</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Appareil de Golgi (virologie)</term><term>Assemblage viral</term><term>Cellules Vero</term><term>Fusion membranaire</term><term>Membrane cellulaire (virologie)</term><term>Microscopie électronique</term><term>Noyau de la cellule (virologie)</term><term>Réplication virale</term><term>Réticulum endoplasmique rugueux (virologie)</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (pathogénicité)</term><term>Vésicules cytoplasmiques (virologie)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Appareil de Golgi</term><term>Membrane cellulaire</term><term>Noyau de la cellule</term><term>Réticulum endoplasmique rugueux</term><term>Vésicules cytoplasmiques</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Membrane</term><term>Cell Nucleus</term><term>Cytoplasmic Vesicles</term><term>Endoplasmic Reticulum, Rough</term><term>Golgi Apparatus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Membrane Fusion</term><term>Microscopy, Electron</term><term>Vero Cells</term><term>Virus Assembly</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Cellules Vero</term><term>Fusion membranaire</term><term>Microscopie électronique</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of the study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Huanying, Zheng" sort="Huanying, Zheng" uniqKey="Huanying Z" first="Zheng" last="Huanying">Zheng Huanying</name><name sortKey="Jicheng, Huang" sort="Jicheng, Huang" uniqKey="Jicheng H" first="Huang" last="Jicheng">Huang Jicheng</name><name sortKey="Jingqiang, Zhang" sort="Jingqiang, Zhang" uniqKey="Jingqiang Z" first="Zhang" last="Jingqiang">Zhang Jingqiang</name><name sortKey="Jinming, Cui" sort="Jinming, Cui" uniqKey="Jinming C" first="Cui" last="Jinming">Cui Jinming</name><name sortKey="Kunpeng, Li" sort="Kunpeng, Li" uniqKey="Kunpeng L" first="Li" last="Kunpeng">Li Kunpeng</name><name sortKey="Ling, Fang" sort="Ling, Fang" uniqKey="Ling F" first="Fang" last="Ling">Fang Ling</name><name sortKey="Xiaojun, Huang" sort="Xiaojun, Huang" uniqKey="Xiaojun H" first="Huang" last="Xiaojun">Huang Xiaojun</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Qinfen, Zhang" sort="Qinfen, Zhang" uniqKey="Qinfen Z" first="Zhang" last="Qinfen">Zhang Qinfen</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005015 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005015 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:15170625
|texte= The life cycle of SARS coronavirus in Vero E6 cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15170625" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |